Development of a New Cell System for the Infectivity Assay of Dengue Viruses
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概要
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A newly established cell line, GK, derived from the kidney tissue of Mongolian gerbils, produced plaques by infection of prototype and wild-type dengue virus strains. Both prototype and wild strains of type 2 virus grew in GK cells and formed plaques at 35.5C and at 31C, while types 1, 3, and 4 wild strains grew and formed plaques only at 31C. In GK cells, plaque formation and the growth of dengue viruses depended on the high (35.5C) and low (31C) incubation temperatures.Virus yields in GK cells of all the 14 dengue virus strains tested, including four prototype and ten wild-type viruses, were 5 to 1, 000-times lower than those in C6/36 cells. After five serial passages in GK cells, types 2, 3, and 4 prototype viruses and type 2 wild strain increased virus yields, and one strain of prototype virus and one strain of wild-type virus decreased mouse neurovirulence.
- 微生物学・免疫学学会連合の論文
微生物学・免疫学学会連合 | 論文
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