Mitogenic Activity of Cytoplasmic Membranes Isolated from L-Forms of <I>Staphylococcus aureus</I>
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概要
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Cytoplasmic membranes of L-forms of <I>Staphylococcus aureus</I> exerted a strong mitogenic effect on splenocytes of athymic nude mice as well as normal mice, while a cytoplasmic fraction of the same bacteria did not show definite mitogenicity. The mitogenic principle(s) of the membrane fraction was resistant to treatment with trypsin and was heat stable (at 100 C for 10 min). The active principle(s) in the insoluble residue of the membrane fraction digested with trypsin was not extracted with cold acetone, but could be solubilized by extraction with a cold chloroform-methanol mixture (2 : 1, v/v). The mitogenic principle(s) in the extract was fractionated by silicic acid column chromatography. Among five fractions separated by chromatography, fractions eluted with chloroform-methanol mixtures (1 : 1 and 1 : 20, v/v) were found to be strongly mitogenic.<BR>The cytoplasmic membranes of the L-forms also exerted a definite mitogenic effect on guinea pig splenocytes, but not on the thymocytes.
- 微生物学・免疫学学会連合の論文
微生物学・免疫学学会連合 | 論文
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