The effects of detergent on the contractility and ultrastructure of frog skeletal muscle.
スポンサーリンク
概要
- 論文の詳細を見る
The contractility of detergent-treated frog skeletal muscle and its ultrastructure were studied. Triton X-100 and Brij-58 were used as nonionic surface active substances. The concentrations of the detergent solutions were adjusted so that the trains of the twitch tensions would continue for about 10min. The duration of the twitch tensions was about 10min when the muscles were soaked in 0.1mM (0.006%) Triton or in 2.5mM (0.280%) Brij solution. The twitch tensions did not recover upon returning the muscles to the normal Ringer solution after treatment of the detergents. Rapid cooling of muscle that had been soaked in a 1.0mM caffeine Ringer solution for 10min provoked a marked contracture (RCC) in 0.1-0.2mM Triton-treated preparation. No such contracture was obtained in 1.0-2.5mM Brij-treated preparation. In ultrastructural findings of the Triton-treated muscle, some structural changes were observed in the transverse tubule and the junctional gap, but not in the terminal cisternae. Brij-treated muscle showed damage of each of these membranes, including the terminal cisternae. These findings suggest that the two detergents have different surface activities on the internal membrane system of the skeletal muscle. The biological activities of the membrane remained even after the treatment of Triton with a suitable concentration.
- 日本生理学会の論文
日本生理学会 | 論文
- 大脳基底核の機能 : パーキンソン病との関連において
- 低温環境下で飼育したカエルの前根電位の変化
- 2,5-ヘキサンジオン投与によるラット会陰筋のミオシン重鎖アイソフォーム成分の変化
- 後肢懸垂によるラットヒラメ筋線維のミオシン重鎖アイソフォーム発現パターンの変化
- 生体における酸素の効率的利用機構