STUDIES ON THE RHODOPSIN OF LIVER EXTIRPATED ANIMALS AND EFFECTS OF CHOLINE ON RHODOPSIN REGENERATION
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概要
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Liver extirpated toads or frogs survived more than 10 days in winter and about 3 days in summer, if they were given glucose and sodium bicarbonate solution after hepatectomy. The increase of bilirubin in serum of the liver extirpated toad was demonstrated by the absorption spectrum of serum.<BR>The change of rhodopsin solution, extracted from the retinae of liver extirpated toads or frogs both in winter and summer, was revealed by a shifting of the point of maximum absorption (λ max.) towards shorter wave lengths 5-10 mμ and therefbre an insufficiency of rhodopsin synthesis could be traced and rhodopsin thereof may be the same as the isorhodopsin described by Collins (3).<BR>When the liver extirpated animals were given choline solution, the point of maximum absorption (λ max.) returned to the normal site.<BR>The yellow color exhibited by the retinae of liver extirpated animals was deeper than that of the control, the velocity of bleaching to visual white being slower. The cause of this phenomenon may be due to the deficiency of retinene reductase, identical with liver alcohol dehydrogenase.
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