STUDIES ON THE SUBSTANCE DERIVED FROM STAPHYLOCOCCUS AUREUS WHICH PROMOTES THE LEUCOCYTES MIGRATION:2) FOLLOWING STUDIES OF L. P. S. BY THE METHOD OF SUBCUTANEOUS AIR CYSTE
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In the previous paper it was reported that Staphylococcus aureus produced the substance promoting the leucocytes migration. And the experimental conditions and method were discussed. In the present paper, the experimental conditions for the purification of L. P. S. was described. The production of L. P. S. by Staphylococcus aureus Terajima, Staphylococcus aureus K. 363 and E. coli was compared. K. 363 was most eminent in the production of L. P. S. and leucocytes migration reached maximum after 4 hours of the injection of K.363. There was no difference in L. P. S. between living and killed bacteria. But the L. P. S. curve with time in the injection of bacteria was qualitatively different from the curve in the injection of glass powder or physiologic saline solution. The possibilty to extract L. P, S. was discussed.
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