MICRODETERMINATION OF POLYAMINES IN BODY FLUIDS AND TISSUES BY HIGH PERFORMANCELIQUID CHROMATOGRAPHY
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概要
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A fully automated, fast and sensitive method has been developed for the analysis of polyamines, their derivatives and various other biogenic amines in body fluids and tissues. Putrescine, spermidine, spermine, cadaverine and unusual polyamines such as norspermidine, norspermine, homospermidine, aminopropylcadaverine and canavalmine can be completely resolved and assayed by high-performance liquid chromatography using a cation-exchange resin with stepwise elution. The column eluate is monitored by a fluorescence detector. It is possible to assay simultaneously other biogenic amines such as histamine, 1-methylhistamine, norephinephrine, normetanephrine and octopamine. Acetylated polyamines can be determined only after their occurrence is confirmed by acid hydrolysis and by other techniques. This method allows the simultaneous determination of these compounds at the picomole level within one hour. It can be directly applied to crude extracts xithout preliminary purification. No pretreatment of samples is required other than deproteinization. This technique could serve as a powerful tool for the diagnosis of malignant tumors and for monitoring the effectiveness of cancer therapy.
- 北関東医学会の論文
北関東医学会 | 論文
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