Lipid peroxidation and antioxidant enzyme status in various tissues of mice with gold-thioglucose-induced obesity.

概要

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To determine whether an oxidant-antioxidant imbalance exists in the non-genetic obese model, we induced obesity in 3-week-old female ICR mice by gold-thioglucose administration, and the lipid peroxide level and activities of antioxidant enzymes were measured in the plasma, liver, heart, and skeletal muscle. Two types of superoxide dismutase were assayed by radioimmunoassays. Lipid peroxide was estimated from fluorimetric measurement of thiobarbituric acid-reactive substances (TBARS). The plasma levels of both cholesterol (Chol) and triacylglycerol (TG) and liver TG content were increased in the obese mice. The superoxide dismutases were unaltered, while the activities of both glutathione peroxidase and catalase in the obese liver and heart were decreased. Lipid peroxide levels were unaltered in the obese liver and heart, decreased in the skeletal muscle, and increased in the plasma. The body weight correlated positively with the liver weight, heart weight, liver TG, liver Chol, plasma TG, plasma Chol, and plasma TBARS, and inversely with the tissue levels of some of the antioxidant enzymes and TBARS. The tissue levels of the antioxidant enzymes and TBARS had a general tendency to decrease in the obese animals. These results suggest that oxidative stress is enhanced only in the plasma of the obese mice and not in any of the tissues studied here.
日本酸化ストレス学会の論文