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A chronic intoxication text on a spirulina product raised by artificial culture at Asahi Carbon Co., Niigata, was carried out by feeding the product to Wistar-strain rats for six months. The rats of both sexes were respectively divided into two groups, and were fed the diet ad libitum; the diet was either a 4:1 mixture of the ordinary diet (N. M. F., Oriental-Kobo Co. Tokyo), dried spirulina or the ordinary diet alone.Comparisons were made between the experimental and control groups in growth and external appearance during the feeding, and in hematological tests and observation of major organs at the end of the experiment. The items of hematological test were Ht, Hb, MCHC, urea-N, serum protein concentration, A/G ratio, concentrations of uric acid, creatinine, total lipids, triglyceride, phospholipids, total cholesterol, total bilirubin, calcium, inorganic phosphate, iron and TIBC, and enzyme activities of ALP, LAP, LDH, SGOT and SGPT. The weight and appearance of organs were observed with regard to the brain, heart, stomach, liver, spleen, kidneys, testes or ovaries, hypophysis and adrenal glands. Histological examination was performed on these organs except the brain, hypophysis and adrenal glands.There were almost no differences between the experimental and the control groups in the growth and external appearance of the whole body or in the shape, weight and histological findings of the organs. Although hematological tests showed some statistical differences in the experiment (p<0.01 by Student's t test), e.g. Hb and SGPT of the male and total bilirubin of the female, abnormal findings were not detected in any items. Accordingly, it may be speculated that the spirulina diet used in this experiment does not bring any toxic effects on the rats within six month feeding.
- 特定非営利活動法人 日本栄養改善学会の論文
特定非営利活動法人 日本栄養改善学会 | 論文
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