Mitochondrial creatine kinase in the extra band of creatine kinase zymogram observed in the fresh sera of healthy subjects.
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概要
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We detected the extra band cathodal to the electrophoretic origin through the analysis of CK isozyme of 346 healthy subjects. This band was observed in all the fresh sera stored at 4°C less than 24h. Biochemical analysis disclosed that neither adenylate kinase nor immunoglobulin-bound CK generated this band. The shift of the extracts migration after the incubation with urea implied that this extra band was derived from mitochondrial CK. We furthermore confirmed the high activation energy of this band (111.75kJ/mol) which was compatible with that of mitochondrial CK. These results strongly support that this extra band was identical with mitochondrial CK. In conclusion presumed mitochondrial CK was observed in the fresh sera of all the healthy subjects. Ordinary cell turnover may be postulated to contribute to this extra band but further study will be needed to define the precise features of this band.
- 日本電気泳動学会の論文
日本電気泳動学会 | 論文
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