Detection of intercellular adhesion molecule-1(ICAM-1) with novel monoclonal antibodies and its significance of malignant diseases.
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概要
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Monoclonal antibody (MoAb) HA 58 (IgG1) was established by immunizing interferon (IFN)-γ treated human colonic cancer BM 314 cells, to BALB/c mouse. Western blotting revealed that the MoAb HA 58 recognizes a 85kD molecule which is recognized by MoAb CL 207 to intercellular adhesion molecule-1 (ICAM-1). Immunodepletion experiment showed that each antibody reacts with the different epitope on the same ICAM-1 molecule. The level of cell surface ICAM-1 expression in cell lines of hematopoietic diseases was high in B-cell lines and especially high in myeloma cell lines. On the other hand, the level was low in T-cell lines and myeloid cell lines, except for ATL cell lines, which showed high level expression. In the investigation of ICAM-1 mRNA expression, the level was parallel to that of cell surface antigen.We established ELISA system for detecting the ICAM-1 antigen by using two MoAbs, CL 207 and biotinylated HA 58. The incidence of positivity for ICAM-1 antigen in malignant diseases was higher than that in benign diseases or in healthy controls. The concentration of ICAM-1 antigen in sera of patients suffering from hematopoietic diseases was high in B-cell leukemia. In cases of B-cell type malignant lymphoma, the concentration was high in cases of advanced stage and/or in cases with leukemic change. As to T-cell malignancies, the concentration of serum ICAM-1 was high in cases of T-CLL, and ATL. The clinical features associated with high level serum ICAM-1 antigen were hepatosplenomegaly, invasion of tumor cells into organs, tumor formation and poor porgnosis.
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