:Production of α1-microglobulin by a hepatoma cell line(C-Hc4)

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α1-microglobulin(α1-m)was detected by radioimmunoassay in the culture medium of a hepatoma cell line (C-Hc4). The level of this protein in the culture medium measured by radioimmunoassay increased proportionately with cell numbers. With the addition of puromycin and cycloheximide to the culture medium, the production of this protein was completely blocked. It was, therefore, suggested that this protein was actively produced by hepatoma cells.This protein showed a complete immunochemical identity with purified α1-m, serum and urinary α1-m from a patient with renal insufficiency by Ouchterlony immunodiffusion and tandem crossed immunoelectrophoresis.In crossed immunoelectrophoresis, this protein migrated at the α1-region, being slightly slower than purified and serum α1-m. On SDS disc electrophoresis combined with immune precipitation, this protein was also found to migrate slightly slower than purified α1-m. These results might be caused by a minor difference of its constituent from that of the purifiedα 1-m during the process of synthesizing this protein in hepatoma cells.
日本電気泳動学会の論文