Simplified assay method for 3-hydroxyanthranilic acid in human urine by fluorometry and its normal value.

概要

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Estimations of human exposure levels to aromatic nitro-and amino-compounds (ANA) are often made by Bratton-Marshall's diazo reaction. In Japan, the environ-mental conditions of the ANA industries have recently improved remarkably. The workers in ANA plants excrete somewhat higher levels of diazotizable substances in the urine than healthy controls and the contributory ratio of the exposed ANA substances to the total diazotizable ones in the urine appears to be of the order of 10 to 20%, most of the time.<BR>Under such circumstances, it is necessary to measure the contributory ratio of physiological ANA substances such as hydroxyanthranilic acid to the total diazotiz-able substances. A simplified assay method for hydroxyanthranilic acid in the urine was devised employing cation exchanging resins for the separation of the hydroxyanthranilic acid from other contaminating substances in the urine, and using fluorometry in alkaline solution for intensifying the fluorescence of hydro-xyanthranilic acid.<BR>The reproducibility of the fluorimetric method was tested by an enzymatic assay method for hydroxyanthranilic acid determination in human urine, and the correla-tion coefficient was satisfactorily high (r=0.890, p<0.01). The normal excretory value of hydroxyanthranilic acid in the human urine ranges from 1.71 to 11.2 (μg/min), which is equivalent to 10 to 70 μmoles/day.
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