Method of Genomic DNA Cloning by the Combination of Cosmid Shuttle Vector and Monoclonal Antibody
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概要
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We report here the strategy to isolate the DNA fragment of any species origin which encodes cell surface antigen by using cosmid library transfection and cell sortings with a monoclonal antibody. We took the mouse melanoma antigen defined by monoclonal antibody as a model system and rescued the genomic DNA by in vitro packaging, showing the feasibility of this procedure.
- 微生物学・免疫学学会連合の論文
微生物学・免疫学学会連合 | 論文
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