Participation of Macrophages in Enhanced In Vitro Immune Interferon (IFNγ) Production with Mouse Spleen Cells
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IFNγ production in cultures of spleen cells obtained from mice sensitized with TH69, a live Streptococcus faecalis preparation, was examined to determine how macrophages participate. It was demonstrated that sensitized spleen macrophages participated in enhanced IFNγ production by T cells at an early stage (0-6hr) of incubation, and that this production is mainly dependent on Ia-bearing macrophages. In the reconstitution experiments where different combinations of spleen macrophages and T cells obtained from mice sensitized with TH69, OK-432, and BCG were used, T cells required that the identity between the sensitizing organisms in vivo and the stimulating organisms in vitro be the same for enhanced IFNγ production while macrophages did not. Macrophage-mediated production of IFNγ appears to be genetically restricted because IFNγ was only produced in cultures where the H-2 region of macrophages and T cells matched.Further examination revealed that for macrophages to participate in enhanced IFNγ production, first contact between cycloheximide-treated macrophages and T cells was required. Second, enhanced IFNγ production occurred when culture supernatants of macrophages obtained from sensitized spleen cells were added to T cells. However, the addition of culture supernatant obtained from sensitized peritoneal macrophages resulted in inhibition of IFNγ production.These results clearly showed the crucial role of macrophages in enhanced IFNγ production by spleen T cells in vitro.
- 微生物学・免疫学学会連合の論文
微生物学・免疫学学会連合 | 論文
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