A New Method for Extraction of Extravasated Dye in the Skin and the Influence of Fasting Stress on Passive Cutaneous Anaphylaxis in Guinea Pigs and Rats
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概要
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A method for quantitative extraction of extravasated dye from the skin was studied in guinea pigs and rats. A simple method with a low cost and good recovery was established as follows; A piece of the skin containing extravasated dye was soaked overnight in a stoppered glass tube containing 1 ml of 1 N KOH at 37 C. Then, 9 ml of a mixed solution of 0.6 N H<SUB>3</SUB>PO<SUB>4</SUB> and acetone (5:13) was added to the tube. The tube was shaken vigorously for a few seconds and centrifuged at 3, 000 rpm for 15 min. Absorbance of supernatant was measured at 620 nm. The recovery rate of the dye was about 95% both in guinea pigs and rats.<BR>Using this method we observed that fasting stress significantly reduced the intensity of skin reactions induced by chemical mediators, heterologous PCA and especially homologous PCA in guinea pigs.
- 微生物学・免疫学学会連合の論文
微生物学・免疫学学会連合 | 論文
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