High Acyl Gellan Networks Probed by Rheology and Atomic Force Microscopy

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概要

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• The network structure of high acyl gellan polysaccharide was investigated using dynamic viscoelasticity and steady flow viscosity measurements, as well as atomic force microscopy (AFM). Time-temperature superposition (TTS) of mechanical spectra of aqueous dispersions having a gellan concentration of 0.1% w/w revealed a gel-like response at the lower end of the frequency range. The TTS master curve of the steady flow data exhibited a power-law relationship between shear viscosity and shear rate at the lower end of the shear rate range, instead of a Newtonian plateau. These rheological characteristics suggest the existence of an effective yield stress arising from the presence of a percolated network. AFM images of high acyl gellan revealed micrometer-sized networks composed of double-stranded helices laterally associated to varying degrees. These associated helices did not dissociate fully on heating at 90°C, suggesting that they are partially preserved native networks secreted by gellan-producing bacteria.

著者

• Ikeda Shinya

  Department Of Applied Biological Chemistry Division Of Agriculture And Agricultural Life Sciences Th

• Nishinari Katsuyoshi

  Graduate School Of Human Life Science Osaka City University

• ZHONG Qixin

  Department of Food Science and Technology, The University of Tennessee

• Gohtani Shoichi

  Department of Applied Biological Science, Faculty of Agriculture, Kagawa University

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