Effect of Hypochlorite-Based Disinfectants on Inactivation of Murine Norovirus and Attempt to Eliminate or Prevent Infection in Mice by Additionto Drinking Water
スポンサーリンク
概要
- 論文の詳細を見る
We evaluated the in vitro efficacy of weak acid hypochlorous solution (WAHS) against murine norovirus (MNV) by plaque assay and compared the efficacy with diluted NaOCl (Purelox) and 70% ethanol. WAHS was as effective as 70% ethanol and diluted Purelox for 0.5-min reactions. For 0.5-min reactions in the presence of mouse feces emulsion, the efficacy of WHAS and 1:600 diluted Purelox was decreased, reducing the virus titers by 2.3 and 2.6 log10, respectively, while 70% ethanol reduced the titer by more than 5 log10. However, WAHS showed more than 5 log10 reductions for the 5-min reaction even in the presence of feces emulsion. Since WAHS showed enough efficacy in inactivating MNV in vitro, we tried to eliminate MNV from MNV-infected mice by substituting WAHS for their drinking water. However, MNV was found to be positive in feces of mice drinking WAHS by an RT-nested PCR and plaque assay. To investigate whether hypochlorite-based disinfectants could prevent infection of a mouse with MNV, WAHS or 1:6,000 diluted Purelox was substituted for the drinking water of mice for 2 or 4 weeks, and then the mice were placed in a cage with an MNV-infected mouse. The supply of disinfectants was continued after cohabitation, but MNV was detected in the feces of all the mice at 1 week after cohabitation. In this study, we tried to eliminate and prevent MNV infection from mice by supplying hypochlorite-based disinfectants as an easy and low-cost method. Unfortunately, drinking disinfectants was ineffective, so it is important to keep the facility environment clean by use of effective disinfectants. Also, animals introduced into facilities should be tested as MNV free by quarantine and periodically confirmed as MNV free by microbiological monitoring.
著者
-
SAKAI Koji
Department of Cardiovascular Medicine, Kyushu University Graduate School of Medical Sciences
-
Tohya Yukinobu
Laboratory Of Veterinary Microbiology Department Of Veterinary Medicine Faculty Of Agriculture Kagos
-
TAHARAGUCHI Motoko
Division of Experimental Animal Research, National Institute of Infectious Diseases
-
Takimoto Kazuhiro
Division Of Experimental Animal Research
-
Yamada Yasuko
Division Of Experimental Animal Research National Institute Of Infectious Diseases
-
Takimoto Kazuhiro
Division of Experimental Animal Research, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan
-
Takagi Hirotaka
Division of Biosafety Control and Research, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan
-
Yamada Yasuko
Division of Experimental Animal Research, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan
-
Tohya Yukinobu
Laboratory of Veterinary Microbiology, Department of Veterinary Medicine, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa-shi, Kanagawa 252-0880, Japan
関連論文
- Long-Term Treatment with Eicosapentaenoic Acid Improves Exercise-Induced Vasodilation in Patients with Coronary Artery Disease
- イヌヘルペスウイルス糖蛋白gD (赤血球凝集素) の性状解析
- ネコヘルペスウイルス1型前初期遺伝子発現機構の解析
- 牛のリポ蛋白中のアポリポ蛋白B (apoB) 濃度 (短報)
- 猫免疫不全ウイルスサブタイプB分離株(TM1およびTM2株)の長期実験感染における遺伝的安定性(短報)(ウイルス学)
- ネコヘルペスウイルス1型赤血球凝集素(gD)発現昆虫細胞のネコ由来培養細胞への接着(短報)
- マレック病ウィルス血清1型感染鶏胚線維芽細胞における糖蛋白Dの検出
- チミジンキナーゼ遺伝子を欠損させた組換え猫ヘルペスウイルス1型の作出
- ネコカリシウイルス日本分離株(FCVF 4株)の非構造蛋白質遺伝子のクローニングと塩基配列の決定
- ネコCD8α鎖を安定に発現する形質転換細胞の作製(短報)
- 精製組換え核タンパク質を用いたELISAによるリンパ球性脈絡髄膜炎ウイルス標準株および分離株に対する抗体の検出
- ネコカリシウイルス野外分離株と広く反応するモノクローナル抗体の作製
- Storage of Gangliosides GM2 and Fucosyl GM1 in the Kidney of MCC Strain of Mastomys (Praomys coucha)
- (免疫学)抗FHV-1およびFCVマウス-猫キメラ抗体の暴露前投与試験
- ネコカリシウイルス野外分離株とモノクローナル抗体のELISAにおける反応性(短報)
- モノクローナル抗体を用いたネコカリシウイルス野外分離株の分類
- 猫CD7遺伝子のクローニング及び塩基配列の決定(短報)(免疫学)
- 猫CD7遺伝子のクローニング及び塩基配列の決定(短報)
- ネコリンパ球表面に存在する白血球共通抗原(CD 45R)に対するモノクローナル抗体の作出
- イヌとネコに由来する各種カリシウイルスのRNAポリメラーゼ遺伝子の比較解析
- 下痢症の犬糞便からの, 猫カリシウイルスに抗原性が交差するカリシウイルスの分離(短報)
- 極性を有するMDCK細胞順化センダイウイルスの病原性
- Enhanced Depressor Response to Endothelial Nitric Oxide Synthase Gene Transfer into the Nucleus Tractus Solitarii of Spontaneously Hypertensive Rats
- Involvement of Rho-kinase in pathogenesis of hypertension : a novel therapeutic target in hypertension
- アルゼンチンにおいて分離されたオーエスキー病ウイルス株の遺伝子型(短報)
- Application of Nested Polymerase Chain Reaction to Detection of Mouse Hepatitis Virus in Fecal Specimens during a Natural Outbreak in an Immunodeficient Mouse Colony
- Contamination of Mouse-adapted Influenza Virus with Sendai Virus
- Analytical Study on Synergistic Effects of Carbonation and Chloride Ion Attack on Concrete
- Prediction of Cracking Effect on tne Pentration of Chloride Ions in Reinforced Concrete
- Chromosomal Mapping and Zygosity Check of Transgenes Based on Flanking Genome Sequences Determined by Genomic Walking
- Serum Biochemical Values in Two Inbred Strains of Mastomys (Praomys coucha)
- Role of Endogenous Rho-kinase in the Nucleus Tractus Solitarii in Regulation of Blood Pressure : Possible involvement of hypertensive mechanism
- Role of endothelium-derived hyperpolarizing factor in human forearm circulation
- Adenovirus-mediated eNOS gene transfer into the nucleus tractus solitarii in mice with heart failure
- Cardiovascular Effects of Overexpression of eNOS in the Rostral Ventrolateral Medulla in Stroke-prone Spontaneously Hypertensive Rats
- Effect of Genetic Background on Establishment of Mouse Embryonic Stem Cells
- Detection of MHV-RNAs in Mouse Intestines and in Filter Dust in Mouse Room Ventilation Duct by a Modified RT-Nested PCR
- Different Baroreflex Responses as a Result of Angiotensin II Produced in Neurons or Glia
- An Integrated Analysis of Reinforced Concrete Beam Subjected to both Loading and Chloride Ion Ingress
- Sequence Analysis of Major Structural Proteins of Newly Isolated Mouse Hepatitis Virus
- Adult onset cardiac dilatation in a transgenic mouse line with Galβ1,3GalNAc α2,3-sialyltransferase II (ST3Gal-II) transgenes: a new model for dilated cardiomyopathy
- Novel Murine Norovirus-Like Genes in Wild Rodents in Japan
- Effect of Hypochlorite-Based Disinfectants on Inactivation of Murine Norovirus and Attempt to Eliminate or Prevent Infection in Mice by Additionto Drinking Water
- β-Ferrocenyl-α,β-unsaturated Ketones. II. The Reduction of β-Ferrocenyl-α,β-unsaturated Ketones and Their Relative Reactivities
- A Novel Reduction in the Presence of Precipitated Metals. II. The Behavior of Precipitated Metals and Water in Catalytic Reduction
- The Catalytic Dehydrogenation, Dehydroxylation and Dehydroxymethylation of Benzyl Alcohol. I. The Scope and the Kinetic Study