Use of Genomic In Situ Hybridization to Identify Chromosomes in Gerbil-Mouse Heterohybridomas
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概要
- 論文の詳細を見る
Heterohybridomas, created to secrete monoclonal antibodies, are generally unstable over long-term culture because of chromosome elimination during culture. To evaluate the karyotype of heterohybridomas, we used simultaneous genomic in situ hybridization (GISH), which can distinguish unambiguously between parental genomes in allopolyploid species. Using GISH, we discriminated gerbil and mouse chromosomes in heterohybridomas with high sensitivity. The GISH technique will allow evaluation of the stability and crossability of heterohybridomas made from the fusion of mouse cells with those of related species.
- Japanese Association for Laboratory Animal Scienceの論文
著者
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Matoba Hideyuki
Department Of Applied Biological Science College Of Bioresource Sciences Nihon University
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Uchiyama Hiroshi
Department Of Applied Biological Science College Of Bioresource Sciences Nihon University
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Kai Osamu
Department Of Animal Science And Resources College Of Bioresource Sciences Nihon University
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Ukaji Takao
Department Of Animal Science And Resources College Of Bioresource Sciences Nihon University
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UCHIYAMA Hiroshi
Department of Applied Biological Science, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan
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KAI Osamu
Department of Animal Science and Resources, College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan
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