Measurement of Transport Activities of 3β-Hydroxy-Δ5-bile Acids in Bile Salt Export Pump and Multidrug Resistance-Associated Proteins Using LC-MS/MS

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概要

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• A method has been developed for the measurement of transport activities in membrane vesicles obtained from Sf9 cells for 3β-hydroxy-Δ5-bile acids by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry. Calibration curves for the bile acids were linear over the range of 10 to 2000 pmol/mL, and the detection limit was less than 1 pmol/mL for 3β-hydroxy-Δ5-bile acids using selected reaction monitoring analysis. The analytical method was applied to measurements of transport activities in membrane vesicles obtained from human multidrug resistance-associated protein 2-, 3-, and human bile salt export pump-expressing Sf9 cells for conjugated 3β-hydroxy-Δ5-bile acids. The present study demonstrated that human multidrug resistance-associated protein 3 vesicles accepted conjugated 3β-hydroxy-Δ5-bile acids along with common bile acids such as glycocholic acid and taurolithocholic acid 3-sulfate.

著者

• Takei Hajime

  Junshin Clinic Bile Acid Institute

• Murai Tsuyoshi

  School of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Kurosawa Takao

  School of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• NITTONO Hiroshi

  Junshin Clinic

• Kimura Akihiko

  Department Of Anatomy School Of Medicine Toho University

• Nittono Hiroshi

  Junshin Clinic Bile Acid Institute

• Oda Kana

  School of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Toyo Terutake

  School of Pharmaceutical Sciences, Health Sciences University of Hokkaido

• Muto Akina

  Junshin Clinic Bile Acid Institute

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