Basic Estimation of Enzymatic Rate Analysis for Determination of Substrate
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Theoretical considerations on enzymatic rate analysis for substrate determination were examined.In the case of enzymatic rate analysis, a dynamic range is decided by Km value of the using enzyme and allowable relative error. When allowable relative error is setted to one per-cent, a dynamic range is less than 1/100Km of the enzyme.Initial velocities are also determined by differential coefficient at zero time of exponential regression formula taken from the reaction time course and the results are in good agreement with the velocities derived from theoretical formula.These theoretical considerations were experimentally established in the system of ammonium ion assay with glutamate dehydrogenase.
- Japan Society of Clinical Chemistryの論文
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