Development and Characterization of Small, Dense LDL Cholesterol Assay Based on Filter Method

概要

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Small, dense LDL has most atherogenic property among various subclasses of LDL. We developed a new and simple analytical method for quantification of small, dense LDL cholesterol, utilizing a separating agent with polyanion and divalent cation natures. As the first assay step, large buoyant LDL, and other apoB-containing lipoproteins (d<1.044g/mL) are aggregated with the separating agent, and are removed through a membrane filter. As the second step, small dense LDL cholesterol obtained is measured enzymatically on a common analyzer. The process enables to actually measure the target analyte even in chylomicron rich samples.<BR>The analytical evaluation studies showed good preci-sion and linearity without any disturbance owing to representative endogenous substances. The comparison studies with other methods indicated good correlations with ultracentrifugation, electrophoresis, NMR and HPLC methods. The best correlation was found with the ultracentrifugation method (r=0.927). The proposed method is easy to perform on a common analyzer, and can be used widely as a routine laboratory method.