On the Analysis of the Interaction of a Biomolecule with its Specific Ligands by Affinity Chromatography

概要

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1. Affinity chromatography was effectively applied to the analysis of complex formation of an enzyme with its specific ligands for the first time.<BR>2. The synthetic procedure for a new support (hydrazide derivative of carboxymethyl-cross-linked dextran) in the preparation of biochemically specific adsorbents is also described. The procedure is based on the hydrazidation of a carboxymethylated polysaccharide via its intra-ester (lactone) derivative.<BR>3. The derived support coupled with some compounds containing primary amino group in a good eff iciency.<BR>4. An analysis of the interaction of carboxypeptidase B with its specific ligands was demonstrated with two kinds of its specific adsorbents which were prepared by the new method.<BR>5. Thq demonstration revealed the multiple ligand-binding sites on the enzyme for small molecular ligands and the cooperative interaction of these sites.
Japan Society of Clinical Chemistryの論文