Simple and Sensitive Method for Measurement of Metoclopramide in Cattle Plasma by LC-MS/MS Using a Multimode Chromatography
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概要
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A quantitative assay method by LC/ESI-MS/MS for the simultaneous determination of MCP in cattle plasma was developed and validated. The chromatographic separation was carried out using a multimode column (2 mm × 150 mm, 3 μm) with gradient elution (0.05% formic acid/methanol with 0.05% formic acid). MCP and levosulpiride (internal standard) were analyzed in the precursor/product ion pair of m/z 300.1/226.9 and m/z 342.0/112.0, respectively. Linear calibration curves were obtained in the range of 2.5–500 ng/ml (R2>0.999) with a lower limit of quantification of 0.05 ng/ml. Mean recoveries were 96–103% and a coefficient of variation was less than 6.5%. Plasma MCP concentrations after 0.4 mg/kg intravenous administration to 12 cattle were determined by the LC-MS/MS method.
著者
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FURUHAMA Kazuhisa
Department of Veterinary Medicine, Faculty of Agriculture, Iwate University
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Kato Masaki
Department Of Chemical Science And Engineering Graduate School Of Engineering Science Osaka Universi
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YAMAGISHI Norio
Department of Veterinary Clinical Medicine, Faculty of Agriculture, Iwate University
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YAMAGISHI Norio
Department of Veterinary Clinical Medicine, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka, Iwate 020–8550, Japan
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HAMAMOTO Kouko
National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1–15–1 Tokura, Kokubunji, Tokyo 185–8511, Japan
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MIZUNO Yasuharu
National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1–15–1 Tokura, Kokubunji, Tokyo 185–8511, Japan
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KATO Masaki
Department of Veterinary Basic Medicine, Faculty of Agriculture, Iwate University, 3–18–8 Ueda, Morioka, Iwate 020–8550, Japan
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FURUHAMA Kazuhisa
Department of Basic Veterinary Medicine, Iwate University
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