Effects of the Expansion Time of Alternating Potential Loading and Temperature on Cell Membrane Damage in HeLa Cells Cultured on an Electrode Surface
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概要
- 論文の詳細を見る
Alternating electrical potentials consisting rectangular pulse waves of 0∼+1.0 V (vs. Ag/AgCl/Sat’d KCl) at 100 Hz were loaded onto MAGI/CCR5 (HeLa-CD4-LTR-β-gal) cells cultured on an ITO electrode. The ratio of cell membrane damage due to electrical potential loading increased with the expansion time of the potential loading, with a positive potential of more than +0.85 V. Furthermore, the induction of cell damage was very sensitive to the temperature of the potential loading. The membrane damage induced by the potential loading was irreversible.
著者
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Harada Shinji
Department Of Applied Chemistry And Chemical Engineering Kagoshima University
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Taniguchi Isao
Graduate School Of Science And Technology Kumamoto University
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Tominaga Masato
Graduate School Of Science And Technology Kumamoto University
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HARADA Shinji
Department of Medical Virology, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University
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KUMAGAI Etsuko
Department of Health Science, Kumamoto University School of Medicine
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NAGAISHI Shoichiro
Graduate School of Science and Technology, Kumamoto University
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