1本のアプタマーによるBound/Free分離システムを用いた血管内皮細胞増殖因子の電気化学的検出系の構築

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概要

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• Vascular endothelial growth factor (VEGF) is expected to find application as a prognostic biomarker in cancer diagnosis. In this study, we measured VEGF using a simple bound/free separation system that utilizes an aptamer, whose capacity for hybridization with capture DNA immobilized on beads changes in the presence or absence of target molecules. Two systems were constructed using a VEGF aptamer selected by in vitro screening and a VEGF aptamer improved by a dimerization strategy. We can detect VEGF based on electrochemistry using both aptamers. We previously reported the detection of two proteins in addition to VEGF—IgE and thrombin—and the success of VEGF detection with this system suggests that this is a versatile system for the detection of various molecules.

• 公益社団法人 電気化学会の論文

著者

• Ikebukuro Kazunori

  Department Of Biotechnology And Life Science Tokyo University Of Agriculture And Technology

• Abe Koichi

  Department Of Advanced Materials Science And Engineering Faculty Of Engineering Yamaguchi University

• ABE Koichi

  Department of Biotechnology, Tokyo University of Agriculture and Technology

• HASEGAWA Hijiri

  Department of Biotechnology, Tokyo University of Agriculture and Technology

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