Ca2+ Waves within Intact Rat Cardiac Trabeculae with Nonuniform Muscle Contraction
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概要
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<B>Background:</B> In the myocardium with nonuniform contraction, Ca<SUP>2+</SUP> waves are initiated not only by spontaneous Ca<SUP>2+</SUP> release from the sarcoplasmic reticulum (SR), but also by dissociation of Ca<SUP>2+</SUP> from the myofilaments. We investigated whether nonuniform contraction affects Ca<SUP>2+</SUP> waves. <B>Methods:</B> Rat trabeculae were regionally exposed to a jet of solution containing 1) 10 mM Ca<SUP>2+</SUP> (Jet<SUB>HCa</SUB>) for the induction of spontaneous Ca<SUP>2+</SUP> release from the SR, and 2) 5 mM caffeine (Jet<SUB>Caff</SUB>) or 0.2 mM Ca<SUP>2+</SUP> (Jet<SUB>LowCa</SUB>) for the regional reduction of muscle contraction. Force, sarcomere length, and [Ca<SUP>2+</SUP>]<SUB>i</SUB> were measured (24°C, [Ca<SUP>2+</SUP>]<SUB>o</SUB>=2.0 mM). <B>Results:</B> The Jet<SUB>HCa</SUB> caused spatially uniform contraction, while the Jet<SUB>Caff</SUB> and Jet<SUB>LowCa</SUB> caused nonuniform contraction. The enhancement of force by the 10% stretch increased the propagation velocity (V<SUB>prop</SUB>) of Ca<SUP>2+</SUP> waves by the Jet<SUB>HCa</SUB>, Jet<SUB>Caff</SUB>, and Jet<SUB>LowCa</SUB>. Interestingly, after preincubation with 10 µM colchicine, this enhancement of force did not increase the V<SUB>prop</SUB> by the Jet<SUB>HCa</SUB>, while it did increase it by the Jet<SUB>Caff</SUB> and Jet<SUB>LowCa</SUB>. The reduction of force by 10 µmol/L blebbistatin did not change the V<SUB>prop</SUB> by the Jet<SUB>HCa</SUB>, while it decreased it by the Jet<SUB>Caff</SUB> and Jet<SUB>LowCa</SUB> irrespective of preincubation with colchicine. <B>Conclusions:</B> These results suggest that even with microtubule-mediated modulation, the force developed during muscle contraction modulates the V<SUB>prop</SUB> in the myocardium with nonuniform contraction.
著者
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Miura Masahito
Department Of Cardiology Tohoku University
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Shindoh Chiyohiko
Department Of Clinical Physiology Health Science Tohoku University Graduate School Of Medicine
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Nagano Tsuyoshi
Department of Clinical Physiology, Health Science, Tohoku University Graduate School of Medicine
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Murai Naomi
Department of Clinical Physiology, Health Science, Tohoku University Graduate School of Medicine
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Miura Masahito
Department of Clinical Physiology, Health Science, Tohoku University Graduate School of Medicine
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