Spectral studies on the cooperative binding mechanism of evans blue to poly(N-vinyl-2-pyrrolidone).

スポンサーリンク

概要

• 論文の詳細を見る

• The binding of Evans Blue (EB) to poly(<I>N</I>-vinyl-2-pyrrolidone) (PVP) was studied at two pH's 7.2 and 11.2 spectrophotometrically by absorbance change method. At pH 7.2, the absorption spectrum of EB changed unusually on adding PVP and this indicated the formation of two types of spectroscopically distinguishable PVP–EB complexes. At very low [PVP], a weak complex was formed which got converted into a strong complex at high [PVP]. Positive cooperativity was found to be operative in this conversion and in the strong complex at higher [PVP]. Scatchard, Hill, and Schwarz methods were employed to analyze this cooperativity and to determine the binding parameters. The analysis revealed: (i) the binding constants for the weak and strong complexes were of the order of 10<SUP>4</SUP> and 10<SUP>5</SUP> dm<SUP>3</SUP> mol<SUP>−1</SUP> respectively; (ii) positive cooperativity at higher [PVP] was due to the interaction of neighboring binding sites on different polymer chains. At pH 11.2, only one type of complex was formed with higher affinity. But the binding behavior remained the same as at pH 7.2.

• 公益社団法人 日本化学会の論文

著者

• Maruthamuthu Meenakshi

  Department of Physical Chemistry, University of Madras

• Subramanian E.

  Department of Physical Chemistry, University of Madras

関連論文

• Bromocresol Green - A hydrophobic spectrophotometric probe for human serum albumin.
• Spectral studies on the cooperative binding mechanism of evans blue to poly(N-vinyl-2-pyrrolidone).

スポンサーリンク