Neutral Salt Effect on the Interaction of Poly-α, L-glutamic Acid with Acridine Orange

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The effect of potassium chloride on the absorption and optical rotatory properties of the poly-α,L-glutamic acid-acridine orange system in an aqueous solution was studied under various conditions. The stability of the helical conformation of poly-α,L-glutamic acid in this system was examined at different potassium chloride concentrations by estimating the helix fraction of the polymer. At pH 4.5, where poly-α,L-glutamic acid exists in the helix, it was found that the added potassium chloride raised the stability of the helical conformation of the polymer in this system. In this case, it was assumed that the added potassium chloride lowers the disturbance of helix formation by the bound acridine orange cation. However, when the solution's pH was raised, the added potassium chloride lowered the extent of the helix fraction in this system. These phenomena were discussed on the basis of several experimental findings on the circular dichroism of the systems. Furthermore, from the change in the intensities of the circular dichroism with the variation in the potassium chloride concentration, it was presumed that the positive circular dichroism band at about 520 nm was due to the binding of dimeric acridine orange molecules to the polymer.
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