Linear-sweep voltammetry and the simultaneous determination of purine bases and their nucleosides in the glassy carbon electrode.

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Adenine, adenosine, guanine, and guanosine were voltammetrically oxidized in a glassy carbon electrode in aqueous solutions, but at different potentials. In general, the nucleosides were oxidized at more positive potentials than their bases. The bases and their nucleosides were strongly adsorbed on the surface of the glassy carbon electrode at the pH values around neutrality, so that the concentration <I>vs.</I> anodic peak current curves were not linear. The adsorption on the electrode was very dependent on the pH, and at pH values below 4 good linear relationships were observed between the anodic peak current and the concentration. The differences between the peak potentials of each of the purine bases and their nucleosides were most pronounced at pH 2–4. Consequently, it was possible to determine simultaneously both in mixtures of the purine bases and their nucleosides by using Britton-Robinson buffer as a supporting electrolyte in the pH range of 2–4. The proposed method is simple and rapid, since no prior treatment or separation procedure is required.
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