Anodic voltammetry and its analytical application to the detection and simultaneous determination of hypoxanthine, xanthine, and uric acid.
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概要
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The voltammetric oxidation of hypoxanthine, xanthine, and uric acid was investigated by means of linearsweep voltammetry, cyclic voltammetry, and controlled-potential coulometry. Hypoxanthine, xanthine, and uric acid each gave a single oxidation peak at a different potential, with a stationary glassy carbon electrode, over the wide pH range of 0–13. Hypoxanthine was initially oxidized in a two-electron step to xanthine, which was then further oxidized in a two-electron step to uric acid, as well as the enzymatic oxidation. All three substances were strongly adsorbed on the surface of the glassy carbon electrode, so that the concentration <I>vs</I> .anodic peak current curves were not linear. The adsorption on the electrode was very dependent on the pH, and especially at pH values around neutrality the anodic peaks were ill-defined. However, in such acid solutions as H<SUB>3</SUB>PO<SUB>4</SUB> and H<SUB>2</SUB>SO<SUB>4</SUB>, the voltammetric oxidation was a diffusion-controlled process and the differences between the peak potentials were most pronounced (<I>ca</I>. 400 mV). Consequently, it was possible to determine simultaneously three hydroxypurine bases by using 1 M H<SUB>2</SUB>SO<SUB>4</SUB> or 1 M H<SUB>3</SUB>PO<SUB>4</SUB> as a supporting electrolyte, without prior treatment or separation procedures.
- 公益社団法人 日本化学会の論文
著者
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Yao Toshio
Department Of Applied Chemistry College Of Engineering University Of Osaka Prefecture
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Musha Soichiro
Department of Applied Chemistry, College of Engineering, University of Osaka Prefecture
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Wasa Tamotsu
Department of Applied Chemistry, College of Engineering, University of Osaka Prefecture
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Taniguchi Yoichi
Department of Applied Chemistry, College of Engineering, University of Osaka Prefecture
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