甲状腺ホルモン分泌調節機構及び血中T<SUB>3</SUB>に関する臨床的研究:第2篇 迅速ラジオイムノアッセイ法による血清T<SUB>3</SUB>測定法ならびにその臨床応用
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A rapid and simple radiommunoassay method for serum T3 was developed using home made potent rabbit anti-T3 serum : <BR>1) The incubation mixture which contained 0.1 ml of 1 : 2,000 diluted anti-T3 serum (finally 1 : 20,000), 250 μg of 8-anilino-1-naphthalene sulphonic acid and 0.1 ml of serum was run at 37°C for 1 hour. Dextran-coated charcoal was added for separation of free and bound T3.<BR>No significant undersirable effects due to high incubation temperature and short incubation time was observed. The lowest detectable amount of T3 was 20 pg per assay though this antiserum showed a 0.24% cross reaction with T4 the purity of which was 99%. ti The dilution curve of serum from Graves' patients was parallel to the standard. The recovery of added T4 over the range 39-625 pg was 98.1%. Inter and intraassay variations of the estimated T3 values at the euthyroid level were 14.3% and 16.9%, respectively.<BR>2) Serum T3 concentrations in 21 normal subjects, 23 patients with Graves' disease, 9 primary hypothyroidism, 19 Hashimoto's thyroiditis were estimated as 131±29 ng/100 ml (mean±s.d.), 546±274 ng/100 ml, 77±27 ng/100 ml and 154±30 ng/100 ml respectively. Calculated T3/T4% in 10 normal subjects was 1.48±0.44, in 21 Graves' patients 2.84±1.59, in 9 primary hypothyroidism 3.88±1.36 and 19 Hashimoto's thyroiditis 2.41±0.86.<BR>3) T3 levels in <SUP>131</SUP>I treated Graves' patients corresponded well with their thyroid functional status. On the other hand their T3/T4% was distributed over a wide range and had no significant relationship with the clinical status.<BR>A hyperbolic inverse correlation between serum T3 and TSH levels (r=-0.21) was found in 88 cases with various thyroid disease, 25 cases of which showed high TSH with normal T3 levels.<BR>A significant increase in serum T3 at 90 and 120 minutes after intravenous administration of 500 μg TRH was observed, and the average of the absolute maximum increment was 42±24 ng/100 ml in 9 normal subjects. It is considered feasible to evaluate both thyroid and pituitary reserve quantitatively by simultaneous estimation of serum T3 and TSH levels after TRH administration.
- 日本内分泌学会の論文
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- 甲状腺ホルモン分泌調節機構及び血中T3に関する臨床的研究:第1篇 固相法ラジオイムノアッセイ法による血清TSH測定法ならびにその臨床応用
- 甲状腺ホルモン分泌調節機構及び血中T3に関する臨床的研究:第2篇 迅速ラジオイムノアッセイ法による血清T3測定法ならびにその臨床応用
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