膵内分泌調節に関する臨床的並びに実験的研究
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Clinical and experimental studies on the insulin-like activity and glucagon-like activity of blood were carried out from the view point of metabolic and neurogenic controls of the endocrine activity of pancratic islets.<BR>Insulin-like activity of blood was determined by Shimazu's rat diaphragm method and glucogon-like activity by Shinko's liver slice method. As the metabolic factors, the following three hexose, i.e., glucose, levulose and galoctose were employed by means of intravenous infusion and by oral administration.<BR>In clinical investigations, normal and diabetic subjects were examined with simultaneous follow-up of blood sugar level. In animal experiments, both normal and alloxan dogs of 7-10kg. body weight were used.<BR>Blood was drawn from V. pancreaticoduodenalis sup., A. femoralis and V. femoralis and blood sugar level was determined by Hagedorn Jensen's method.<BR>The results are summarized as follows ;<BR>1) In normal and diabetic subjects per oral administration of glucose induced an increase of insulinlike activity of blood. The one hour percent increase of this activity in normal subjects was more pronounced than that of diabetics.<BR>The diabetics who do not require insulin showed higher percent increase of insulin-like activity than the diabetics under insulin treatment.<BR>2) Following intravenous infusion of glucose solution insulin-like activity of blood showed transient decrease and then turned to increase in normal subjects. The author has assumed, that this indicates a possible acceleration of insulin secretion by an initial consumption of blood insulin by infused glucose. In diabetics this transient decrease of insulin-like activity disappered with increasing severity of the disease, showing direct increase immediately following glucose infusion.<BR>The glucagon-like activity of blood of normal subjects showed a transient increase in 30 minutes after infusion of glucose solution but returned to preinjection level in 60minutes.<BR>In diabetics, however, with the increasing severity of the disease, this normal pattern of glucagon was lost. It decreased transiently at first and then increased upon glucose infusion.<BR>3) Investigations on normal and alloxan dogs in case of intravenous infusion of glucose solution revealed almost the same pattern as normal subjects and diabetics as regard to the insulin-like activity of blood. The pattern of blood insulin-like activity showed slight modification following the insulin consumption in the blood.<BR>Glucagon-like activity of blood showed no particular change both in normal and alloxan dogs. In general, one can say that alloxan dogs tend to secrete glucagon rather slowly than normal dogs.<BR>After infusion of glucose solution an increase of A-V ratio was observed, resulting most plausibly from the increased glucose uptake in the peripheral tissue under the co-influence of insulin and glucagon.<BR>4) In case of infusion of galactose solution blood glucose decreased parallel to the increasing value of the total blood hexose.<BR>There was a similar patten of insulin-like activity and glucagon-like activity of blood as in the case of glucose infusion.<BR>Thus, galactose seems to promote glucagon secretion, insulin consumption and subsequent insulin secretion. A-V ratio also showed increase on galactose infusion.<BR>5) No paticular change of insulin-like activity or glucagon-like activity of blood was observed on levulose infusion. This seems to indicate that insulin is not necessarily required for metabolism of levulose.<BR>Total hexose of blood showed no corresponding increase after levulose infusion. This seems to indicate the relatively quick break down of levulose after infusion. A-V ratio showed no increase on levulose infusion.<BR>6) Electrical stimulation of Vagal Nerve brought about no regular increase or decrease of insulinlike activity of blood drawn from V. pancreaticoduodenalis sup..
- 日本内分泌学会の論文