Wistar系ラツトの糖尿病持続期間とその子のLangerhans氏島β細胞発育障害との関係について

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It was reported by K. Okamoto and his coworkers that disorders in the development of β-cells in Langerhans' islets are seen in offsprings obtained by mating rats of Wistar strain which had alloxan-diabetes for a long period. T. Tanaka reported on the duration of diabetization required in Wistar male rats to bring about damage of β-cells in their offsprings. To investigate the duration of diabetization required in female rats to cause a decrease in the number of β-cells in their offsprings and to investigate something further in male or female rats, the following experiments were done. a) Wistar female rats with diabetic state induced by application of alloxan were mated with normal male rats after various durations of diabetization. b) Females which, after having diabetes for over 40 days, were treated with insulin for varying lengths of time and were mated with normal males. c) Males manifesting glycosuria for a period of 23 days, during which one-or two-day insulin treatment was given at various times were mated with normal females. d) Females with glycosuria lasting for 24 days, which received insulin treatment either on the 1st or on the 14th day, were mated with normal males. e) Normal males and females, whose Langerhans' islets were ascertained to be normal by an exploratory partial resection of the pancreas, were mated with each other. Thereafter the males were made alloxan-diabetic for over 40 days, and then mated with the same females that they had been mated with earlier. The offsprings thus obtained, weighing over 150 g at the 90th day after birth, were sacrificed by ether. The Gomori's staining was applied on the pancreas, and histometrical examination was done on the number of β-cells in Langerhans' islets. The results obtained are summarised as follows : 1) In 30 normal rats (15 males, 15 females) the number of β-cells in one islet was found to be 65.4-70.4 (67.7±1.5 in average), the number of α-cells 14.0-19.6 (15.6±1.8 in average). There was no difference between males and females. 2) In experiment a), all of the young rats obtained by mating normal males and females, which had been in a diabetic state for 22 days (6 cases) or 23 days (10 cases), had the normal number of β-cells. The number of β-cells in all of the young, obtained from females with diabetes lasting for 24 days (6 cases), 25 days (6 cases), 26 days (5 cases), 27 days (5 cases) or over 40 days (10 cases), was decreased, the average number of β-cells being 58.3±0.78, 57.8±0.79, 58.3±1.1, 58.7± 0.75, 58.5±0.81, respectively. It might be concluded, therefore, that the diabetic state lasting for a period of the last 24 days of oogenesis, has an effect on female sexual cells that brings about a decrease in the number of β-cells in their offsprings. 3) In experiment b), the number of β-cells in all of the young obtained from females which showed no glycosuria for 3 days after 40 day diabetization (10 cases), was decreased (59.0±0.94 in average). In all of the young, obtained from females aglycosuric for 4 days (8 cases), 5 days (10 cases), 6 days (8 cases) or 7 days (9 cases), the number of β-cells was normal. It is indicated that certain changes in oocytes, responsible for the decrease in the number of β-cells in the offsprings, disappear when the non-diabetic state lasts for the period of the last 4 or more days of oogenesis. 4) In expersment c), in all of the young obtained by mating normal females with males showing glycosuria for a period of 23 days, during which insulin treatment was given either on the 1st or on the 14th day (10 cases and 19 cases, respectively), the number of β-cells was normal. When the males were treated on the 2nd day, 9 of their 18 young were normal, the average number of β-cells being 67.1±1.4,
一般社団法人日本内分泌学会の論文

著者

田口富子
京都大学医学部病理学教室

Wistar系ラツトの糖尿病持続期間とその子のLangerhans氏島β細胞発育障害との関係について

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