尿中ACTH新定量法及びその臨床知見

概要

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A new benzoic acid absorption method and Sayers, Sayers, Woodbury's bioassay method was successfully used for quantitative determination of urinary ACTH in healthy subjects as well as patients with pituitary-adrenal disorders and under synthetic steroids therapy. The recovery experiments showed about 76% by this method. In 25 normal subjects the adrenal ascorbic acid depletion in hypophysectomized rat (AAD) caused by 26 extracts from 24 hr urine was 51±6.5 (M±SE) mg/adrenal 100g, while ten samples corresponding to 12 hr urine from 4 patients with Addison's disease gave highly significant increase of the AAD (p<0.001). In both hypopituitarism (12 samples in 7 cases) and patients under steroid therapy (18 samples in 12 cases), significant decreases were noted (the former aver. 22±8.7 p<0.02, the latter aver. 15±7.9p<0.01). In 5 cases of anorexia nervosa, 5 samples showed no significant difference both from normal or hypopituitarism. In 10 samples from 6 cases of Cushing's syndrome with conclusive evidence of adrenal hyperplasia only 2 cases gave high ADD values, and one case with unilateral adenoma showed low AAD, followed by normal level postoperatively. Possible explanations for this are discussed. It was concluded that our new method of urinary ACTH determination was of great clinical usefulness.
日本内分泌学会の論文