横隔膜法に依る血中Insulin測定と糖尿病の本態に関する検討

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1) The author investigated about the measurement of serum insulin activity, on the basis of Gemmill's observation, that the isolated rat diaphgram uptakes glucose in the glucose containing solution and the glucose uptake is increased by the addition of insulin to the solution. 2) Wistar strain rats were bred about for 7 days with solid food (Oriental Co. made) under the condition of temperature 22±5°C, and were abstained from the food for 15 hours before experiment. 3) These rats were sacrificed by decapitation, and the diaphragms were immediately removed, each of them were dissected in 4 pieces. So-called “quartardiaphragm technique of pooled method” was used for this investigation. 4) Phosphate buffer solution containing 200mg/dl. sugar was used for medium. For the incubator, the author used the manometer flask of Warburg's apparatus. The flask contains one-fourth of diaphragm, medium and 95-100% O2, incubated for 75 minutes at 38°C, 90 rounds shaking per minute. 5) Glucose uptake of diaphgram per 1 gram (dry weight) in the medium before and after incu-bation, the mean values of four quartardiaphgrams from four rats were used. This value means basal uptake. The difference between glucose uptake in the medium containing test solution and basal uptake means extraglucose uptake. When used insulin solution of known concentration to medium, this difference called as “insulin effect”. 6) It became clear from the experiment, that the relation between insulin effect (Y) and insulin.concentration (X) could be expressed by means of next equation. log Y=3/10 log X + log 40 7) Insulin value (insulin activity in sera) in normal subjects and diabetic patients, resulted as follows. normal subjects 0.339±0.08 mu/ml. diabetic patients 0.125±0.115mu/ml. normal rabbits 0.355±0.125mu/ml. alloxan diabetic rabbits 0.00 -082mu/ml. normal dogs 0.158±0.032mu/ml. pancreatectomized dogs 0.00-<0.01 mu/ml. these values were the averages of 5-10 cases. 8) The remainings of injected insulin in blood of diabetic patients retarded than those by normal bodies, and moreover, these insulin in blood still retained its activity. Same results were obtained from the experimental diabetic animals. 9) Glucose uptake and insulin effect of the diaphragm from alloxanized rat decreased than those of controls. 10) From the result described in 7), 8), and 9) the author proved that insulin in blood decreased on diabetic patients, and diabetes mellitus is not caused by decreasing of activated insulin, but rather by decreasing of insulin utilizing ability. 11) Glucagon decreases glucose uptake and insulin activity in peripheral tissues, and so it acts as insulin inhibitor.
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横隔膜法に依る血中Insulin測定と糖尿病の本態に関する検討

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