カテコラミン定量法の進歩
スポンサーリンク
概要
- 論文の詳細を見る
1. Various chemical and biological methods for the determination of catecholamines, particularly in biological materials, were summarized and criticized.<BR>2. Using Amberlite IRC-50 column, it was first demonstrated that dopa, is excreted in normal human urine and that its rate of excretion exceeds that of adrenaline.<BR>3. Certain neutral and acidic catechols which develop fluorescence by ethylenediamine condensation method were separated from urine and their properties were discussed. It was confirmed that one of these catechols is 3, 4-dihydroxyphenylacetic acid.<BR>4. Euler & Floding (1955) and others determined the concentration of urinary adrenaline and noradrenaline using the following procedure; the catechol fraction is isolated by alumina adsorption and then adrenaline and noradrenaline are estimated by trihydroxyindole method. But this procedure should not be applied here, because it neglects the occurence of dopa in urine which gives a positive reaction to trihydroxyindole method.<BR>5. Weil-Malherbe & Bone (1957) purified further the alumina eluate of urine by passage through a cation exchange resin, but dopa was not eliminated by this procedure.<BR>6. It is assumed that Amberlite IRC-50 adsorption technique may be most excellent, because the resin retains the basic fraction exclusively and eliminates other catechols (dopa, neutral and acidic catechols etc.).<BR>7. Weil-Malherbe & Bone (1957) reported that there were no other catechols except adrenaline and noradrenaline in normal (bovine) serum. But there is no evidence that other catechols do not appear under pathological conditions.
- 日本内分泌学会の論文