Rapid Identification of Mycoplasma pulmonis Isolated from Laboratory Mice and Rats Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

スポンサーリンク

概要

• 論文の詳細を見る

• Mycoplasma species identification is based on biochemical, immunological, and molecular methods that require several days for accurate identification. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a novel method for identification of bacteria and has recently been introduced into the clinical microbiology laboratory as a rapid and accurate technique. This method allows a characteristic mass spectral fingerprint to be obtained from whole inactivated mycoplasmal cells. In this study, we evaluated the performance of the MALDI-TOF MS for the identification of Mycoplasma by comparison with standard sequence analysis of 16S rRNA. We developed the first database of MALDI-TOF MS profiles of Mycoplasma species, containing Mycoplasma pulmonis, M. arthritidis, and M. neurolyticum, which are the most common pathogens in mice and/or rats, and species-specific spectra were recorded. Using the database, 6 clinical isolates were identified. Six tracheal swabs from 4 mice and 2 rats were cultured on PPLO agar for 4 to 7 days, and the colonies were directly applied to analyze the protein profiles. Five strains were identified as M. pulmonis, and 1 strain from a mouse was identified as M. neurolyticum (spectral scores were >2.00); the results were consistent with the results of the 16S rRNA gene sequence analysis (homologies>97.0%). These data indicate that MALDI-TOF MS can be used as a clearly rapid, accurate, and cost-effective method for the identification of M. pulmonis isolates, and this system may represent a serious alternative for clinical laboratories to identify Mycoplasma species.

著者

• Tamura Takashi

  Graduate School Of Engineering Tokyo Metropolitan University

• HAYASHIMOTO Nobuhito

  Central Institute for Experimental Animals

• Goto Kazuo

  Department Of Cardiology Jichi Medical School

• MAKIMURA Koichi

  Graduate School of Medical Technology, Teikyo University

• ASAHARA Miwa

  Graduate School of Medical Technology, Teikyo University

• MATSUMURA Mitsuru

  Department of Clinical Laboratory Medicine, School of Medical Technology, Teikyo University

• YAMAMOTO Mikachi

  Laboratory of Space and Environmental Medicine, Graduate School of Medical Technology, Teikyo University

• HAYASHIMOTO Nobuhito

  Central Institute for Experimental Animals, ICLAS Monitoring Center

関連論文

• 実験感染および自然感染マウスからのマウスノロウイルス核酸の検出
• THE INFLUENCE OF THE REGIONAL MYOCARDIAL CONTRACTION ON THE INITIAL LEFT VENTRICULAR IMPULSE : Ischemic Heart Disease : PROCEEDINGS OF THE 50th ANNUAL SCIENTIFIC MEETING OF THE JAPANESE CIRCULATION SOCIETY
• -155-INTERDEPENDENT RIGHT AND LEFT VENTRICULAR/VASCULAR COUPLING
• Higher Yielding Isolation of Kinsenoside in Anoectochilus and Its Antihyperliposis Effect
• Characteristic karyotype abnormalities including Robertsonian translocations found in eight mouse embryonic stem cell lines
• Clinical and Histopathological Evaluation of Dermatophagoides farinae-Induced Dermatitis in NC/Nga Mice Orally Administered Bacillus subtilis
• Morphological and Sequence Analysis of Mycoplasma sp. Isolated from the Oral Cavity of a House Musk Shrew (Suncus murinus)
• Expression of a spinach gene encoding a cysteine synthase-like protein in tobacco plants
• Retroperitoneal Malignant Fibrous Histiocytoma Causing Variegated Colonic Lesions
• Clinical Experience with Expanded Polytetrafluoroethylene Sheet Used as an Artificial Dura Mater
• The Role of Cysteine 116 in the Active Site of the Antitumor Enzyme L-Methionine γ-Lyase from Pseudomonas putida
• Discrepancy inequalities of Erdos-Turan and of Le Veque
• Heterotopic Pancreas in the Stomach which Caused Obstructive Stenosis in the Duodenum
• The Angular Distribution of α-Particles from the Reaction ^F(pα)^O^*(π)^O
• The Role of Amino Acid Residues in the Active Site of L-Methionine γ-lyase from Pseudomonas putida
• The Role of Amino Acid Residues in the Active Site of L-Methionine γ-lyase from Pseudomonas putida
• Rapid Identification of Mycoplasma pulmonis Isolated from Laboratory Mice and Rats Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
• Effect of Tilted Edges on Coupled Vibrations of Doubly Rotated Quartz Plates

もっと見る 閉じる

スポンサーリンク