Cross-Reactivity of Chicken Anti-Japanese Encephalitis Virus Serum and Anti-West Nile Virus Serum in Serological Diagnosis
スポンサーリンク
概要
- 論文の詳細を見る
The cross-reactivity of Japanese encephalitis virus (JEV)-immunized chicken sera and West Nile virus (WNV)-immunized chicken sera in serological tests, such as IgG indirect ELISA (IgG-ELISA), hemagglutination inhibition test (HI) and plaque reduction neutralization test (PRNT) of JEV and WNV were examined. The mean of JEV/WNV ELISA ratio in IgG-ELISA of JEV-immunized sera was significantly higher than that of WNV-immunized sera (P<0.01). JEV-immunized chicken sera did not cross-react in WNV HI. However, all the WNV-immunized chicken sera cross-reacted in JEV HI. JEV-immunized chicken sera did not show the WNV neutralization titer at 90% plaque reduction, and WNV-immunized chicken sera did not showed the JEV neutralization titer at 90% plaque reduction. Therefore, it is possible that chicken JEV serum is distinguished from WNV serum by comparing the titers of IgG-ELISA, HI or PRNT respectively.
著者
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SHIBAHARA Tomoyuki
National Institute of Animal Health, National Agriculture and Food Research Organization
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HIROTA Jiro
National Institute of Animal Health, National Agriculture and Food Research Organization
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SHIMIZU Shiya
National Institute of Animal Health, National Agriculture and Food Research Organization
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KOBAYASHI Sota
National Institute of Animal Health, National Agriculture and Food Research Organization
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SHIMIZU Shinya
National Institute of Animal Health, National Agriculture and Food Research Organization
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SHIBAHARA Tomoyuki
National Institute of Animal Health, 3-1-1 Kannondai, Tsukuba, Ibaraki 305-0856, Japan
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HIROTA Jiro
National Institute of Animal Health
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- Cross-Reactivity of Chicken Anti-Japanese Encephalitis Virus Serum and Anti-West Nile Virus Serum in Serological Diagnosis
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