葉酸欠乏症および再生不良性貧血における核酸合成
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概要
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Rats were raised on a folic acid-free ration, and with the folic acid-deficient rats so obtained as material, next experiments were made on folic acid metabolism. The biosynthesis of nucleic acids and proteins in the bone marrow of patients of hypoplastic anemia was studied, and by comparing the results of the study with the findings in the experiments with the folic acid-deficient rats, the etiology of hypoplastic anemia and the significance of massive dose folic acid therapy were discussed.<BR>As the result of the author's experiments, it was revealed that, in the folic acid-deficient rats, the DNA biosynthesis was most markedly impaired, and the RNA biosynthesis was less but still markedly impaired, but the protein biosynthesis was only slightly impaired. In patients of hypoplastic anemia, the DNA biosynthesis was not virtually impaired, but the RNA and protein biosynthesis were impaired. The activity of DNA-dependent RNA polymerase was markedly low in both the folic acid-deficient rats and the patients of hypoplastic anemia. The assay by the L. casei method revealed that the folic acid level in the serum was low in the patients of hypoplastic anemia. These findings led to a surmise that the template activity is reduced by the formation of abnormal DNA in folic acid deficiency, and the de novo synthesis of purines in the liver is impaired. The DNA activating area was narrowed in the bone marrow of patients of hypoplastic anemia, and it is undeniable that massive dose folic acid therapy will possibly normalize the area.
- 学校法人 昭和大学・昭和医学会の論文