人体表皮の組織培養における栄養液の研究:第I報: 栄養液のpHとそれが表皮細胞の増殖におよぼす影響について

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As a part of the studies on the nutrients for the tissue culture of epidermal cells, the author investigated the influence of the each constituent of nutrient and its pH with special emphasis on the influence for the tissue culture of human epidermis. 1, pH of human serum collected under usual condition without being air-tight was 8.0±0.1 2. pH of the chick embryo extract diluted with the same quantity of Ringer's solution was 6.5±0.06. 3. pH of the nutrient composed of salt solution (Ringer), human serm and chickembryo extract in a ratio of 4 : 4 : 2 was 7.8±0.08. 4. Nutrient adjusted of its pH by the addition of N/10 HCl and N/10 KH2PO4 practically showed no change in provided the nutrient was hermetically sealed. While, when. CO2 gas was used, the pH of nutrient showed a tendency to increase. 5. The nutrient containing N/10 HCl and N/10 KH2PO4 in a concentration of 10% inhibited the membraneous outgrowth of the epitherial cell. Increase in the concentration.of CO2 gas, likewise, inhibited the membraneous outgrowth of the epitherial cell. 6. Epitherial cell showed poor proliferation in the nutrient of high pH concentration This poor growth of epitherial cell might also due to the toxic charactor of HCI, KH2PO4. or CO2 gas used for the adjustment of pH.
昭和大学・昭和医学会の論文