ペプチドハイドロゲルを用いた3次元モデル人工真皮の作製

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Recently regenerative medicine has made enormous progress, and various artificial skins are being developed as composite cultured skin. Among them, hybrid artificial skin contains living fibroblasts that are cultured on a scaffold derived from animals, such as cows, pigs and rats. Such material from animals inevitably contains the potential risk of infection, from agents both known and unknown. Therefore, we have developed a three-dimensional (3D) model cultured dermis based on non-biogenic peptide hydrogel scaffolding. To make the cultured dermis, human dermal fibroblasts derived from newborns were mixed with the peptide hydrogel, dispensed into the insert, and cultivated for a maximum of 2 weeks in D-MEM. We evaluated samples of the cultured dermis by HE staining, AZAN staining, and immunohistochemistry staining for human type I collagen. The fibroblasts appeared foam-like evenly distributed in the 3D structure of the peptide hydrogel. In addition, the presence of human type I collagen confirmed by immunocytochemistry suggests that the fibroblasts in the peptide hydrogel were functional. Peptide hydrogel consists of standard amino acids (1%) and 99% water. Under physiological conditions, the peptide components self-assemble into a 3D hydrogel that exhibits a nanometer-scale fibrous structure. Peptide hydrogel is a synthetic matrix that is used to create defined 3D microenvironments for a variety of cell culture experiments. Peptide hydrogel is biocompatible, resorbable, and devoid of animal-derived material and pathogens. We have developed a model three-dimensional (3D) cultured dermis using peptide hydrogel as a scaffold with the potential for clinical application. In this study, we investigated the method to produce cultured dermis with peptide hydrogel as the scaffold, and demonstrated the survival and functional manifestation of fibroblasts in peptide hydrogel.
学校法人昭和大学・昭和医学会の論文

ペプチドハイドロゲルを用いた3次元モデル人工真皮の作製

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