ブタ水晶体上皮細胞を用いた紫外線傷害モデルの検討

概要

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The aim of this study was to investigate the 50% lethality of monochromatic ultraviolet (UV) radiation on porcine lens epithelial cells (LEC) . Porcine LECs were isolated and cultured in Dulbecco's Modified Eagle Media (DMEM) -F12 supplemented with 10% fetal bovine serum (FBS) and antibiotics. Confluent monolayers of LEC in 24-well tissue culture plates were incubated for 24 h with DMEM-F12 containing 0.2% FBS for synchronization of the cell cycle (G0) . During 280, 291, 301 or 309 nm UV light irradiation, the medium was changed to Dulbecco's PBS (-) which showed 100% transmittance of UV light. The 50% lethality of each wavelength was determined by living cell counts at 24 hr after irradiation: 1.4 mJ/cm2 at 280 nm, 3.2 mJ/cm2 at 291 nm, 13 mJ/cm2 at 301 nm, and 200 mJ/cm2 at 309 nm, respectively. These data would provide a clear index for the LECs cell injury by spectroscopy.
学校法人昭和大学・昭和医学会の論文