実験的膝関節外傷の修復に関する研究 主として滑膜組織肥胖細胞の変動について
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Since the report of Ehrlich in 1877 on the presence of the tissue mast cells, a variety of facts have so far been clarified on their functions, but a number of points remain yet to be clarified.<BR>Recently, a hypothesis is presented of Lindholm et al., in which the tissue mast cells are regarded as calcium transporter. So I have experimentally explored whether or not the tissue mast cells of synoival membrane (TMC) which are most closely related to the joint with no periostal membranes have the similar functions as they repoted.<BR>An experiment was conducted on young, male Wistar strain rats, 250 to 300 gram in weight, under etherial anesthesis, in which the animals were allocated to 4 groups according to the procedure employed for the production of experimental knee joint traumata.<BR>1. Group with curettage of joint cartilage and without fixation.<BR>2. Group with curettage of joint cartilage with its intramedullary fixation with Kirachner's wire.<BR>3. Group without curettage of joint cartilage and with its intramedullary fixation with Kirschner's wire.<BR>4. Group with mannual lesion knee joint without fixation.<BR>Each group had 8 sub-group divided for each week covering the period from the fourth day to the end of the seventh week.<BR>Tetracycline for i.v. was intraperitoneally injected into all the experimented rats 24 hours before their victimization. After victimization, soft tissue was taken with each of the ex-perimented rats and their knee joints were extirpated. From on animal out of each group, non-decalcified triturated specimens were prepared for subsequent contact-microradiography (MRG) and assay with tetracycline labeling (TcL) . All the other animales were used for preparation of specimens decalcified and embedded with paraffin, which were used for stain-ing with toluidineblue at pH 4.1, with PAS, and with haematoxylin-eosin (H-E) in their microtomed specimen forms.<BR>Taking into consideration the roentgenographic and macroscopic examination thus made, in addition to the histo-pathological examination, TcL, and MRG, the behaviors of TMC in the knee joint traumata, the relationship between calcium and TMC, and the pathological pattern of the synovial membranes were explored to find the following results.<BR>1) The TMC decreased, or disappeared, at the acute inflammation stage, but gradually increased at it entered chronic stage. Metachromasia also showed various patterns but the TMC decreased as the tissues were gradually returned to normal. Metachromasia also became weaker in parallel to it.<BR>2) The TMC of the control group was found to have increased generally beyond that the nontraumata group, regardless of the degree of the traumata or the period after receiving the traumata. The reason for this has yet to be clarified, but the traumata are likely to affect somehow the changes in the TMC values over the whole body.<BR>3) Positive findings with TcL and MRG not detected with the synovial membranes. There was no finding that would histo-pathologically insinuate growth of new bones. Therefore, no possible function as the calcium transporter was found out with the TMC.<BR>4) Pale yellow fluorescence was observed using TcL at ossification center, curettage location of bones and newly created part of bones, <BR>5) The restoration of intra-articular fracture was made with the uniting callus and the sealing callus, and pannus also was contributory to the above restoration.
- 学校法人 昭和大学・昭和医学会の論文