An analytical study on the mechanism of Coenzyme Q10 enhancement of the effect of Adriamycin in cultured mouse sarcoma cells.

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It is said that Coenzyme Q10 augments the inhibition of DNA synthesis of cultured mouse sarcoma cells (Meth A cell) produced by Adriamycin in a dose dependent manner.To elucidate this enhancing mechanism, we analyzed the intracellular concentrations of Adriamycin in Meth A cells and Swiss albino 3T6 immortalized cells by flowcytometry techniques. In Meth A cells, a large part of Adriamycin's action is due to an active transport system, especially in influx. The intracellular concentration of Adriamycin rises with increasing doses of Coenzyme Q10. Thus, the augmenting antitumor effect of Coenzyme Q10 depends on its rapid increase and on the maintenance of a high intracellular concentration of Adriamycin. On the contrary in 3T6 cells, the level of Adriamycin decreases with increasing doses of Coenzyme Q10, because active efflux is more enhanced by the presence of Coenzyme Q10 than active influx, while passive influx and active efflux are the main actions of Adriamycin in the usual state without Coenzyme Q10.It is concluded that Coenzyme Q10 plays an opposite role in tumor cells and normal cells by the different way and it affects the transport mechanism of Adriamycin. These findings suggest that combination chemotherapy with Coenzyme Q10 and Adriamycin is more effective, not only because it prevents adverse reactions such as cardiopathy, but also because it augments the anticancer effect of Adriamycin.
耳鼻咽喉科臨床学会の論文