Several Factors Affecting Ozone Gas Sterilization

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Ozone gas sterilization was confirmed using commercially available as well as homemade biological indicators (Bls) utilizing Geobacillus stearothermophilus (formerly it was classified as Bacillus stearothermophilus) ATCC 12980 or ATCC 7953. The relationship between the ozone gas concentration, relative humidity or temperature and decimal reduction value (D) of Bl was studied. We also investigated how the ozone gas sterilization performance was affected by the difference in the characteristics of the carrier materials of Bls. The carrier materials used were synthesized polymers with different characteristics and stainless steels (SUSs) with different levels of surface roughness and cleanness. Effect of the difference in the ATCC number of the BI on the D values was also studied. There existed a satisfactory relationship between temperature, humidity and ozone gas concentration and D value (r>0.9). Results indicated that D values from Bls with carrier materials of more hydrophilic materials were greater. In addition, the D value of the Bl with a carrier material made of SUSs was greater than that made of polymers. The difference in the roughness of the surface of the SUS material did not cause any significant difference in D values. On the contrary, the difference in the cleanness caused a difference in D values. The difference in the SUS components, i.e. Fe, Ni, may cause some differences in D values. Resistance of G. stearothermophilus ATCC 12980 was around two times greater than that of ATCC 7953 when expressed as CT (concentration X time).

Several Factors Affecting Ozone Gas Sterilization

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