わが国の沿岸海域における炭化水素酸化細菌に関する生態学的研究-1-炭化水素酸化細菌の計数法

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Little has been known as yet of the ecology of hydrocarbon-oxidizing bacteria in Japanese coastal waters such as the Inland Sea where various petrolic and organic pollutants are abundantly poured. Therefore, a series of studies has been undertaken to make it clear. To start with, some methods to enumerate hydrocarbon oxidizers in these areas have been examined in this paper, and results obtained can be summarized as follows. As suitable organic and nitrogen sources for hydrocarbon oxidizers in the surveyed regions, L-alanine and ammonium chloride are recommended respectively. Each liquid medium in which the said suitable organic or inorganic nitrogen source, the basal salt solution and the appropriate hydrocarbon substrate are contained, is tentatively named Medium A or B, and then hydrocarbon oxidizers which are cultured in Medium A or B is designated Group A or B, respectively. The approximate numbers of hydrocarbon oxidizers of both groups in samples if marine materials are enumerated by means of minimum dilution technique, and their accurate numbers are enumerated by means of the minimum dilution technique, and both cases hydrocarbon oxidizers are determined to be existent on the basis that the substrate in the cultured fluid decreases remarkably, which was measured by a gas chromatographic procedure. Emulsification of the fluids incubated in both media, and the decrease in pH value of the fluid in only Group B, are recognized as the supplementary criteria for indicating the existence of hydrocarbon oxidizers in the inocula such as sea water and bottom sediments.
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わが国の沿岸海域における炭化水素酸化細菌に関する生態学的研究-1-炭化水素酸化細菌の計数法

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