ブリ血漿アルブミンおよびグロブリンの分離〔英文〕

概要

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Albumin and globulin fractions were isolated from the plasma of yellow tail, Seriola quinqueradiata, by the classical salting-out method. Physico-chemical analyses showed that the albumin fraction is composed of several components possessing relatively higher electrophoretic mobilities and lower molecular sizes than the globulin components. The dominant albumin component was a kind of lipoglycoprotein, with a molecular weight between 160, 000 and 180, 000, which possessed a BPB-binding ability and a high mobility. In addition, none of the albumin components was found to fit completely the definition of mammalian serum albumin. The molecular weights of the globulin components were more than 250, 000. The presence of plural lipoproteins was observed in the globulin fraction as well as in the albumin fraction. Several methods of evaluating the albumin/globulin ratio were compared. Dye-binding methods were inadequate with yellow tail plasma. On the other hand, the values obtained by the cellulose acetate electrophoresis method and the salting-out method were found to correlate with a correlation coefficient of +0.43 (n=50).
日本水産學會の論文