海域における有機物の無機化について〔英文〕

概要

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A simplified method for the determination of rate of mineralization of organic matter is devised by using a diffusion technique instead of an aeration method. The apparatus is a 50ml amber glass Erlenmyer flask with an inner chamber. In the inner chamber, Hyamine solution in methanol and an accordion-folded piece of filter paper are placed, and in the outer chamber, microorganisms collected on a Millipore HA filter by filtering sea water, the filtrate and uniformly [14C] labelled substrate are added. After sealing tightly with a serum rubber cap, the reaction is initiated in a motor-drive incubator. The reaction is stopped by adding acid through the rubber cap with a syringe. Further incubation is continued to accomplish the absorption of CO2 into the inner chamber. The radioactivity of [14C] CO2 derived from the mineralization is measured by a liquid scintillation spectrometer. The error of this method was under 10% caluculating from the 25 pairs of experiments. The mineralization rate was estimated by applying this method to field surveys in Beppu Bay.
日本水産學會の論文