マダコ後唾腺蛋白分解酵素の消化への関与に関する研究-5-後唾腺蛋白消化酵素の探索
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In the first paper of this series it was confirmed by biological experiments that the greater number of the proteinase and the carboxypeptidase A (CPA)-like enzymes in the digestive tract originate from the posterior salivary glands and greatly contribute to digestion. Some investigations, then, were undertaken in order to confirm the fact mentioned above. Seven proteinases (Enzymes I to VII) and CPA (Enzyme VIII) were separated from extracts of the glands and were purified, but no identical enzyme with the proteinase of the digestive juice could be found in these seven proteinases. Therefore, it has been impossible to find out which proteinase has a digestive role in the digestive tract. The present paper deals with an examination for finding protein digestive enzymes in the glands. Between an octopus that fasted all day and one sampled 30 minutes after feeding, there was a remarkable difference in the composition of proteolytic enzymes of the glands. From the results of the above investigation, it was suggested that the following three enzymes are the protein digestive enzymes of the glands. They are chymotrypsin A (Ch A)-like enzyme (Enzyme I), CPA (Enzyme VIII), and the CPA-like enzyme (Enzyme IX) which has not been confirmed so far to exist in the glands. In addition, it has already become clear that Enzyme VIII is the CPA which has a di-gestive role in the digestive tract and that Enzyme I differs from the Ch A-like enzyme of the digestive juice in physicochemical properties but agrees closely with it in enzymic properties.
- 日本水産學會の論文
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