ドジョウの卵巣濾胞内で起こるプロテア-ゼ分泌について(魚類の排卵機構に関する研究-4-)

概要

論文の詳細を見る
An attempt was made to detect the ovulation-inducing enzymes, which digest the envelopes of ovarian follicles, using loach Misgurnus anguillicaudatus as material. Ovarian follicles both just before ovulation (taken from HCG-injected females) and far from it (taken from saline-injected females) were placed in contact with the same gelatin-silver film and incubated in moist chamber for five hours at 37°C simultaneously. Only the former was able to digest gelatin completely in the area of contact, showing that active secretion of protease(s) had occurred just before ovulation. Considering that most proteases have some ability of rupturing follicles, these protease(s) detected in this experiment might act as ovulation-inducing enzymes. On the other hand, ovarian fluid in which various-sized follicles were immersed seemed to have no proteolytic activity.